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Of interest is the strict conservation of the four cysteines (Cys14, Cys47, Cys101 and Cys169) that confer peculiar redox sensitivity to this enzyme

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Use adaptive dosing and staging matched to disease phase and compartment: for example, early ischemiareperfusion may benefit from acute, time-boxed control of mitochondrial bursts and labile iron to limit Fenton chemistry, while chronic vascular or organ remodeling calls for rebalancing NOX activity and ER stress together with lifestyle re-entrainment (exercise, nutrition, sleep, exposure control) to reset redox tone without erasing physiological H 2 O 2 signalling (Poznyak et al